ox42 monoclonal mouse anti ratcd11b antibody  (Bio-Rad)

Journal: Surgery Open Science

Article Title: Protective effect of HDACIs in improves survival and organ injury after CLP-induced sepsis

doi: 10.1016/j.sopen.2023.03.003

Figure Lengend Snippet: Effects of isoform-specific and non-selective HDACIs on CLP-induced sepsis immune cell response. The spleens were homogenized into single-cell suspension and stained with anti-rat CD3, B220, CD11b, GR-1 and F4/80. Flow cytometry analysis was conducted on the different cell populations. Left representative of plots T lymphocytes, B lymphocytes, innate immune cells, macrophages and neutrophils are shown. Histograms on the right show relative changes in the indicated cell population. * P < 0.05, ** P < 0.01 and *** P < 0.001 for significant differences between the treatment and VEH groups. Sham: the peritoneal cavity was opened, but the cecum was eviscerated without ligated or punctured; VEH: vehicle; TubA: tubastatinA; TSA: trichostatin-A;

Article Snippet: The monoclonal antibodies were as follows: CD3 monoclonal antibody (1F4) (Thermo Fisher, product # MA5-16461), CD45R (B220) monoclonal antibody (HIS24) (eBioscience , product # 12-0460-82), F4/80 monoclonal antibody (BM8) (Santa Cruz, product # SC-377009), CD11b/c monoclonal antibody (OX42) (eBioscience, product # 12-0110-82) and anti-Ly-6G/Ly-6C (Gr-1) (RAT) fluorescein-conjugated monoclonal antibody (Rockland, product # 200-502-L54).

Techniques: Staining, Flow Cytometry

Journal: Journal of Neuroinflammation

Article Title: LPA 1 signaling drives Schwann cell dedifferentiation in experimental autoimmune neuritis

doi: 10.1186/s12974-021-02350-5

Figure Lengend Snippet: Minor impact of AM095 treatment on immune cell infiltration. Immune cell infiltration was assessed by counting the number of CD3 + (T-lymphocytes) and CD11b/c + (myeloid cells) on sciatic nerve sections in randomly selected fields. a Representative images of immune cell infiltration at peak of disease at 14 dpi, and early remission phase at 21 dpi. b + c No significant difference was observed in the number of infiltrating CD3+ cells between peak of disease and remission phase ( N = 9/8/4/4 from left to right). d + e While there was no significant reduction in CD3 + cells at 14 dpi, we recognized a slight decrease in myeloid cells under AM095 at 21 dpi ( N = 5/5/5/6 from left to right)). Cell counts were normalized to area. Data represent mean ± s.e.m. P ≤ 0.05*. Scale bars indicate 50 µm

Article Snippet: The following antibodies were used at the indicated dilutions: rabbit monoclonal [SP7] anti-CD3 (abcam; diluted 1:500); mouse monoclonal [1F4] anti-CD3 (BioLegend; diluted 1:1000); mouse monoclonal [OX42] anti-CD11b/c (abcam; diluted 1:1000); rabbit polyclonal anti-Sox2 IgG (abcam, ab97959; diluted 1:200); rabbit monoclonal [EPR4007] anti-Sox10 (abcam; diluted 1:250).

Techniques:

Journal: Journal of Immunology Research

Article Title: Acute Neuroinflammatory Response in the Substantia Nigra Pars Compacta of Rats after a Local Injection of Lipopolysaccharide

doi: 10.1155/2018/1838921

Figure Lengend Snippet: Time course of microglial activation. (a) A representative photograph of a Western blot membrane showing the electrophoretic fractionation of OX42 and β -actin from substantia nigra homogenates of LPS-treated rats and untreated (Ut) control rats. The numbers indicate the time of evaluation. (b) Graph of densitometry analysis showing the normalized values of OX42 bands concerning β -actin bands. The values represent the mean ± SD ( n = 3 independent rats in each time of each experimental condition). ∗ P < 0.001 when compared with the untreated control group using repeated-measures one-way ANOVA and Newman-Keuls post hoc test. (c) Representative micrographs of the double immunofluorescence of TH and OX42 in the substantia nigra of untreated (Ut) control rats and rats at different times after LPS injection that were taken at 3.8 mm from the interaural midpoint on the dorsal-ventral axes of the rat brain atlas by Paxinos and Watson . The numbers at the left side of micrographs indicate the time of evaluation. Immunofluorescence (IF) area density for TH (d) and OX42 (e) was determined using ImageJ software v.1.46r (National Institutes of Health, Bethesda, MD). The TH and OX42 values for the mock rats correspond to the quantification in Supplementary . All values represent the mean ± SD ( n = 3 independent rats in each time of each experimental condition). ∗ P < 0.001 when compared with the untreated control group of the respective immunostaining. † P < 0.001 when compared with the respective mock group. Repeated-measures two-way ANOVA and Bonferroni post hoc test.

Article Snippet: The primary antibodies were mouse monoclonal anti-tyrosine hydroxylase (TH) (clone TH-2) (1 : 1000; Sigma-Aldrich, St. Louis, MO, USA), rabbit polyclonal anti-TH (1 : 1000; Millipore, Temecula, CA, USA), mouse monoclonal anti-CD11b/c (OX42) (1 : 200; Abcam, Cambridge, UK), mouse anti-CD45 (BD Bioscience, USA), and rabbit polyclonal anti-GFAP (1 : 500; DakoCytomation, Glostrup, Denmark).

Techniques: Activation Assay, Western Blot, Membrane, Fractionation, Control, Immunofluorescence, Injection, Software, Immunostaining

Journal: Journal of Immunology Research

Article Title: Acute Neuroinflammatory Response in the Substantia Nigra Pars Compacta of Rats after a Local Injection of Lipopolysaccharide

doi: 10.1155/2018/1838921

Figure Lengend Snippet: Morphological changes during activation of microglia in the substantia nigra after LPS exposure. Representative confocal micrographs of OX42 immunofluorescence in the substantia nigra of untreated control rats and rats at different times after LPS injection.

Article Snippet: The primary antibodies were mouse monoclonal anti-tyrosine hydroxylase (TH) (clone TH-2) (1 : 1000; Sigma-Aldrich, St. Louis, MO, USA), rabbit polyclonal anti-TH (1 : 1000; Millipore, Temecula, CA, USA), mouse monoclonal anti-CD11b/c (OX42) (1 : 200; Abcam, Cambridge, UK), mouse anti-CD45 (BD Bioscience, USA), and rabbit polyclonal anti-GFAP (1 : 500; DakoCytomation, Glostrup, Denmark).

Techniques: Activation Assay, Immunofluorescence, Control, Injection

Journal: Journal of Immunology Research

Article Title: Acute Neuroinflammatory Response in the Substantia Nigra Pars Compacta of Rats after a Local Injection of Lipopolysaccharide

doi: 10.1155/2018/1838921

Figure Lengend Snippet: Apparent phagocytosis of damaged dopaminergic neurons by microglia. Representative confocal micrographs of TH and OX42 double immunofluorescence. (a) Integrated projections from x-y optical stacks. (b) Orthogonal projections from a 1 μ m z-confocal optical section. The arrows show a green fluorescence dot (TH immunoreactivity) surrounded by a red fluorescence ring (OX42). The right top panel corresponds to the integrated image where the orthogonal analysis was performed.

Article Snippet: The primary antibodies were mouse monoclonal anti-tyrosine hydroxylase (TH) (clone TH-2) (1 : 1000; Sigma-Aldrich, St. Louis, MO, USA), rabbit polyclonal anti-TH (1 : 1000; Millipore, Temecula, CA, USA), mouse monoclonal anti-CD11b/c (OX42) (1 : 200; Abcam, Cambridge, UK), mouse anti-CD45 (BD Bioscience, USA), and rabbit polyclonal anti-GFAP (1 : 500; DakoCytomation, Glostrup, Denmark).

Techniques: Immunofluorescence, Fluorescence